Microinjections
Today was a much simpler day, but we encountered a lot more
problems. Group A and group B are done with
microinjections until CTA testing. We
gave microinjections only to group C today.
Of group C3, rats in Box 1 and Box 2 received 0.3M NaCl, and rats in Box
3 and Box 4 received 0.5M NaCl. Group C1
rats received 0.05M saccharine, and group C2 rats received 0.3M MSG. For group C3, all aCSF injections went
well. However, the first rat receiving
CDP had to be reinjected on both sides.
The second rat receiving CDP was reinjected on the left side twice for
the air bubble to move in the left tubing.
Two rats from group C1, one receiving aCSF and one receiving CDP, had to
be reinjected for the air bubble to move.
We were behind schedule today due to the many difficulties with the
microinjections. Julia and I agitated
the cannulae of the rats in group A and group B. We also gave the rats in group D water for
one hour.
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