Microinjections
Today, we continued microinjections for group C. The first round of microinjections went well
for group C3, and they were all given .01M saccharine. We gave water to group D
for 60 minutes while group C3 was testing.
A problem we encountered a few times was that the air bubble went down,
but not to the point where the correct amount of CDP was injected. In this situation, we cannot give them
another dose of the drug, so we have to test them with what was actually injected.
Group C1 rats in Box 1, Box 2, and Box 3 received 0.1M MSG, and the rat in Box 4 received .01M saccharine. The air bubble in the left tube of one rat in
group C1 and one rat in group C3 did not move, so the left side was
reinjected. When the injector was taken
out of the left side for the rat in group C2, it began to bleed a little so we
agitated his cannula after he tested. All rats in group C2 received .1M MSG. All other injections were good. Only one rat did not have many licks. Group C was given water for 15 minutes after all of them had
been tested, and we agitated the group D rats’ cannulae.
No comments:
Post a Comment