Day 12

Microinjections

We ran group D rats today, starting with group D2.  The first two rats’ microinjection ran well, but the second rat receiving CDP had to have a second microinjection on the left side.  When I inserted his left dummy back in his cannula, a little liquid was coming out.  All rats in group D received 0.01M quinine.  I gave 60 minutes of water to group C while group D2 was running.  For group D3, all aCSF microinjections went well.  The first rat receiving CDP had to have two microinjections, and the second rat receiving CDP had to have three microinjections.  I changed out the bedding for the D2 rats while they were running and took out the trash with Alex.  For group D1, all aCSF microinjections went well, and both rats receiving CDP had to have two microinjections on the left side for the air bubble to move in the left tubing. 

CTA Testing

Julia and I prepared four syringes with LiCl to use for any rats that not react aversely to the NaCl.  They would need to be reinjected with the LiCl after drinking the NaCl to establish an aversive relationship.  We encountered some problems when preparing the syringes.  Some needles did not securely fit on the syringes, and we had more trouble today than yesterday getting rid of the air bubbles in the LiCl solution.  It took longer, but we finally managed to properly prepare the four syringes.