The first set of microinjections went well for group
C1. However, the second rat receiving
CDP had problems. He received three
microinjections on the right side, and the air bubble still didn’t move in the
tubing. We decided to run him today anyways
and retest him another day as well. We
also had trouble with the rats in group C2 receiving CDP. The first rat had three microinjections, but
thankfully the second rat only needed one microinjection for the air bubble to
move. All rats in group C1 and group C2
received 0.01M quinine. Group D rats
were given 60 minutes of water. While
group C2 was testing, I prepared jars with formalin. I labeled 17 jars total with the subject
number of each rat in group A and group B.
I then filled the jars about 2/3 full with formalin. We ran group C3 rats, and only had to
reinject the first rat receiving CDP once before the air bubble moved. Rats in Box 1, Box 2, and Box 3 received
0.01M quinine while the rat in Box 4 received 0.03M NaCl. One rat only had one lick. Some rats in group A and group B were
retested for CTA. The other rats of
group A and group B who successfully experienced taste aversions the previous
day were finished testing. I was able to
see the preparation for tissue sampling performed by Dr. Holstein and Dr.
Pittman.
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