Day 5

Microinjections

We did more microinjections today on Group A again.  Each rat was placed in the same Box in the testing room as they were placed in before, but this time the rats received the opposite treatment (those with CDP last time were given aCSF, and those with aCSF last time were given CDP).  We continued to agitate the cannulae of the rats that were not being tested.  One problem encountered during microinjections was that the air bubble did not move in the tube on the right side.  When the air bubble doesn’t move, it means the drug is not being injected.  The injection was attempted again using the left tubing, but the air bubble still did not move.  The injector was cleaned and reinserted into the tube.  This time the microinjection was successful (the problem was most likely due to a clogged injector).  This put us back a little on time, but everything else ran smoothly.  We ended the day again by giving 15 minutes of water to the rats.