Day 6

Microinjections

Today was another day of microinjections.  Group A was tested.  They received the same drug as they did the first time they were tested and are placed in the same box, however, Box 1 and Box 2 received the .003M citric acid and Box 3 and Box 4 received the .01M sucrose.  I also vortexed the drugs before they were used to make sure the solution was completely mixed.  Group B was given water for 60 minutes and their cannulae were agitated.  For one rat, we had the same problem that we did the other day.  It took three tries until the air bubble finally moved for CDP in the right side.  The drug was delivered using the left tubing.  We also had to replace a few injectors because the rats moved while they were being put in, causing them to bend or break.  At the end of the day, we cleaned the cages, gave group A 15 minutes of water, and relabeled the baby bottles with numbers one through four.